Coding

Part:BBa_K5048068:Design

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-24)


Neae-LAP-6xHis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1990
    Illegal PstI site found at 3427
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1990
    Illegal PstI site found at 3427
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1990
    Illegal BglII site found at 4470
    Illegal BglII site found at 4568
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1990
    Illegal PstI site found at 3427
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1990
    Illegal PstI site found at 3427
    Illegal NgoMIV site found at 2676
    Illegal NgoMIV site found at 3942
    Illegal AgeI site found at 2308
    Illegal AgeI site found at 3964
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1244
    Illegal SapI site found at 4290


Design Notes

We constructed this part by restriction endonuclease cleavage and T4 ligation. During the process of design, we had to consider the enzyme and the cleavage site we needed to use. When inserted into the pET-28a backbone, this part is included in Adhesion Construction(Fig 1).


Fig 1 The genetic circuit respectively for pET-28a-[Neae-LAP] (right)

Source

See our parts: BBa_K5048047, BBa_K5048058


References